The Fly-RRF-36 has support for connecting Neopixels using the header called “rgbled” (GPIO26).
Neopixels can be wired to the Fly-RRF-36 as shown below.
The Neopixels should be configured in config.g using M950 as detailed here.
For example, using the default toolboard address of 124 with RGBW Neopixels
M950 C"124.rgbled" E0 T2
M150 can then be used to set the colours and brightness.
For example, the below example will set 3 RGBw neopixels to white.
M150 E0 R0 B0 U0 W255 S3 F0